P-80: The Effect of Alkaline Environments Made with Sodium BiCarbonate in order to Separate Spermatozoa for Sex Selection

Background: There are different factors Tahat effect in sex selection such as the best medium treatment.These techniques can be used in live stock industry for product in ordre to humans need. Materials and Methods: Forty mice (NMRI) were divided in to a control group and three experimental group. Ovulation of mice was stimulated by using PMSG and HCG hormones. Female mice were killed by cervical dislocation and dissect. Oocytes were collected from the oviduct. Spermatozoa from the cauda epididymis were getting and entered on media by pH (7.5, 7.6, 7.7). The faster sperms used for IVF. Embryos of control groups and exprrimental group were transferred into foster mother and after birth, male and female infants were counted. Analysis of data was done by Spsss, Anova,softwere. Results: The proportion of male and female in control group(pH=7.4), experimental group 1 (pH=7.5) and experimental group2 (pH=7.6) and experimental group3 (pH=7.7) was (48.61), (65.99), (67.33), (68.49) (Mean±SD) respectively. The comparison of proportion of male to female infant between control group and experimental groups showed significant differences (p <0.05). But there was not significant difference among experiment groups 2,3 with conrol group. Conclusion:This research showd that sperm activity in Y chromosome increased with alkaline invironments and Sodium Bicarbonate.